Hyaluronate Biotin - Items tagged as "Fluorescein"

Biotinylated Hyaluronate (bHA) is a high-performance affinity probe designed for sensitive detection and characterization of hyaluronan-binding proteins. The biotin tag enables strong and specific interaction with streptavidin- or avidin-based detection systems, allowing visualization of hyaladherins such as CD44, RHAMM, cartilage link protein, aggrecan, versican, and neurocan at nanogram sensitivity (≥2 ng).

bHA performs reliably on nitrocellulose following SDS-PAGE and is ideal for electroblotting, receptor-binding analysis, extracellular matrix profiling, and studies of HA-mediated cell signaling, migration, and tissue remodeling. This reagent provides a robust, reproducible method for identifying HA-binding domains and validating HA–protein interactions in developmental, regenerative, and cancer biology research.

References

1. Biotinylated hyaluronic acid: a new tool for probing hyaluronate-receptor interactions. Bioconjug Chem., 1994, 5(4):370–372.

2. Biotinylated hyaluronic acid as a probe for identifying hyaluronic acid-binding proteins. Anal Biochem., 1995, 228(2):299–306.

3. Biotinylated hyaluronan: a versatile and highly sensitive probe capable of detecting nanogram levels of hyaluronan-binding proteins (hyaladherins) on electroblots by a novel affinity detection procedure. Electrophoresis, 1996, 17(1):205–212.